Bottom Line Up Front
The BioFlo 120 is the bridge between iterative R&D and manufacturing readiness. It is the fastest and most cost-effective way to generate a robust data package and ensure your process is ready for pilot-scale.
At a Glance
- Equipment type: Bench-scale, autoclavable fermenter
- Working volume: 0.8 to 2.2 L
- Controls: Temperature, pH, dissolved oxygen
- Additions: Up to 4
Use Case
Process de-risking & scale-up validation
Growth Curve Bio uses the BioFlo 120 to enable efficient technology transfer and upstream process development. By running multiple bench-scale fermentations in parallel, we quickly test control versus experimental conditions and ensure process robustness before moving up to pilot-scale. This results in the most efficient use of time and money for our clients.
Why BioFlo 120?
- Operational flexibility: Suitable for batch, fed-batch, and fill and draw processes
- Precise control: 4 peristaltic pumps enable complex, highly-controlled feeding and inducing strategies
- Real-time data: Industry-standard sensors constantly monitor temperature, pH, dissolved oxygen, and foam
Key Inputs
- Culture: Client-provided strain / cell line
- Media: Defined or complex media recipe
- Operational parameters: Setpoints for temperature, pH, and dissolved oxygen, along with dissolved oxygen cascade (agitation, aeration, supplemental gas)
Key Outputs
- Precise data: High-resolution logs of temperature, pH, dissolved oxygen (and components of the dissolved oxygen cascade)
- Analytical material: Fermentation broth for offline analytics (at GCB, client site, or third-party lab)
- DSP material: Fermentation broth for bench-scale downstream processing
- Scalable protocol: Recipes and control logic that are ready for pilot-scale vessels
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